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rat glp 1r plasmid  (Addgene inc)


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    Structured Review

    Addgene inc rat glp 1r plasmid
    Rat Glp 1r Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+glp+1r+plasmid/pET11b-MIF+H62Y+(Plasmid+%23149442)/pmc06843041-182-12-25
    Average 90 stars, based on 1 article reviews
    rat glp 1r plasmid - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Does glucagon-like peptide-1 induce diuresis and natriuresis by modulating afferent renal nerve activity?
    Article Snippet: .. Human embryonic kidney (HEK)-293 cells seeded with 60% confluence were transfected with rat GLP-1R plasmid (plasmid catalog no. 14944, 2.5 μg/well in a 12-well plate, Addgene, Watertown, MA) or empty pcDNA3 plasmid as its corresponding negative control vector using Opti-MEM and Lipofectamine 3000 (Life Technologies, Carlsbad, CA) as transfection reagents. ..

    Plasmid Preparation:

    Article Title: Does glucagon-like peptide-1 induce diuresis and natriuresis by modulating afferent renal nerve activity?
    Article Snippet: .. Human embryonic kidney (HEK)-293 cells seeded with 60% confluence were transfected with rat GLP-1R plasmid (plasmid catalog no. 14944, 2.5 μg/well in a 12-well plate, Addgene, Watertown, MA) or empty pcDNA3 plasmid as its corresponding negative control vector using Opti-MEM and Lipofectamine 3000 (Life Technologies, Carlsbad, CA) as transfection reagents. ..

    Article Title: Structural Modeling and in Silico Screening of Potential Small-Molecule Allosteric Agonists of a Glucagon-like Peptide 1 Receptor
    Article Snippet: HEK293 cells stably expressing the CRE/CREB luciferase reporter gene (BPS Bioscience #60515), RPMI medium (Corning #10-040), KRB (Amsbio #KRB-1000), l -glutamine (Gibco #25030-081), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco #15630-080), sodium pyruvate (Gibco #11360-070), β-mercaptoethanol (MP #806444), d -glucose (#G-7528), fetal bovine serum (Fisher #03600511), penicillin/streptomycin (Corning #30-002-Cl), hygromycin B (Alfa Aesar #J60681), lipofectamine 2000 (Invitrogen #11668027), VIPR peptide agonist (Sigma #V3628), VIPR peptide antagonist (Sigma #SCP0260), GLP-1R peptide agonist (Sigma #9416), 6-well cell culture plates (Ultra Cruz #sc-204443), 96-well cell culture plates (Sigma #CLS9102), luciferase cell culture lysis reagent (Promega #E1531), luciferase assay reagent (Promega #E1501), and Ultra-Sensitive Rat Insulin Kit (Crystal Chem #90060) were purchased from vendors. .. Rat GLP-1R plasmid (#14944) and VIPR1 plasmid (#51865) were purchased from Addgene, Flag-tagged Human GLP-1R and Flag-tagged pCMV-N-Flag negative control vector were purchased from vendors (Sino Biological Inc. #HG13944-NF and #CV061), and pcDNA3.1 vector and INS-1 832/13 cells were kindly provided by Dr. Xianxin Hua (University of Pennsylvania). ..

    Negative Control:

    Article Title: Does glucagon-like peptide-1 induce diuresis and natriuresis by modulating afferent renal nerve activity?
    Article Snippet: .. Human embryonic kidney (HEK)-293 cells seeded with 60% confluence were transfected with rat GLP-1R plasmid (plasmid catalog no. 14944, 2.5 μg/well in a 12-well plate, Addgene, Watertown, MA) or empty pcDNA3 plasmid as its corresponding negative control vector using Opti-MEM and Lipofectamine 3000 (Life Technologies, Carlsbad, CA) as transfection reagents. ..

    Article Title: Structural Modeling and in Silico Screening of Potential Small-Molecule Allosteric Agonists of a Glucagon-like Peptide 1 Receptor
    Article Snippet: HEK293 cells stably expressing the CRE/CREB luciferase reporter gene (BPS Bioscience #60515), RPMI medium (Corning #10-040), KRB (Amsbio #KRB-1000), l -glutamine (Gibco #25030-081), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco #15630-080), sodium pyruvate (Gibco #11360-070), β-mercaptoethanol (MP #806444), d -glucose (#G-7528), fetal bovine serum (Fisher #03600511), penicillin/streptomycin (Corning #30-002-Cl), hygromycin B (Alfa Aesar #J60681), lipofectamine 2000 (Invitrogen #11668027), VIPR peptide agonist (Sigma #V3628), VIPR peptide antagonist (Sigma #SCP0260), GLP-1R peptide agonist (Sigma #9416), 6-well cell culture plates (Ultra Cruz #sc-204443), 96-well cell culture plates (Sigma #CLS9102), luciferase cell culture lysis reagent (Promega #E1531), luciferase assay reagent (Promega #E1501), and Ultra-Sensitive Rat Insulin Kit (Crystal Chem #90060) were purchased from vendors. .. Rat GLP-1R plasmid (#14944) and VIPR1 plasmid (#51865) were purchased from Addgene, Flag-tagged Human GLP-1R and Flag-tagged pCMV-N-Flag negative control vector were purchased from vendors (Sino Biological Inc. #HG13944-NF and #CV061), and pcDNA3.1 vector and INS-1 832/13 cells were kindly provided by Dr. Xianxin Hua (University of Pennsylvania). ..



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